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Hypophysiotropic factor (HIF), a non-prolactin hypothalamic factor released in association with suckling, stimulates the proliferation of rat pituitary lactotropes. We have prepared a rat hypothalamic cell line (HrLT-14) that synthesizes and secretes HIF and that retains all the characteristics of the pituitary lactotropes in terms of morphology, GH-releasing factor (GRF) and somatostatin (SRIF) receptors, GRF-binding capacity, and cytosolic calcium responses to GRF and SRIF. The factor is released basolaterally, as measured by the accumulation of 125I-GRF in the medium, and HIF release corresponds temporally to the changes in cytosolic calcium in response to GRF and SRIF. These data and the location of GRF receptors on the cell surface suggest that HIF may act within the hypothalamo-pituitary axis and through stimulation of GRF receptors rather than by diffusion.1. Field of the Invention
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